Lambda Light Chains Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50129 Clone# BP6133 Predicted Molecular Wt: 23kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P0CG04 Background: Lambda light chains are polypeptide chains located in the cell membrane and cytoplasmic regions of normal B cells and plasma cells. The combination of lambda light chains and heavy chains forms immunoglobulin molecules. There are two classes of light chains found in immunoglobulins, kappa light chains and lambda light chains. Light chain production by lymphoid cells is genetically restricted such that the immunoglobulin molecules produced by an individual cell will only contain a single light chain class. The normal human kappa/lambda ratio is approximately 2:1. The presence of clear cut light chain restriction with a kappa/lambda ratio more than 10:1 is consistent with a malignant proliferation. This antibody recognizes lambda light chains of human immunoglobulins, which may be useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Subcellular location: Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to residues within N- terminus of Immunoglobulin lambda constant 1 was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Kremer M,et al. Virchows Arch. 2005 Dec; 447(6):920- 37. 2. Marshall-Taylor CE, et al. Appl Immunohistochem Mol Morphol. 2002 Sep; 10(3):258-62. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of tonsil tissue labelling Lambda Light Chains with BP6133. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
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