IgD Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50130 Clone# BP6134 Predicted Molecular Wt: 42kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P01880 Background: Immunoglobulins are produced by cells of the B-lymphocyte lineage. Based on differences in the heavy chain, five immunoglobulin isotypes are known as IgA, IgG, IgM, IgDand IgE. Human IgD exists in two forms: secreted IgD (secIgD), present in small amounts in human serum, and membrane-bound IgD (mIgD), present on the surface of mature B cells. MIgD is co-expressed with membrane-bound IgM (mIgM) and plays a major role as an antigenic receptor on the surface of B- lymphocytes. IgD is expressed in normal and neoplastic mantle B cells. It is absent in most cells of normal splenic marginal zone but present in 30% to 40% of splenic marginal zone lymphomas (MZLs). Additionally, IgD may be a marker for the identification of nodular lymphocyte predominant hodgkin lymphoma. Antibody to IgD is useful for classification of B-cell derived lymphomas and plasmacytomas. Subcellular location: Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to residues within aa50-150 of Human IgD was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Leong AS, et al., Geenwich Medical Media Ltd. 1999; 217-219. 2. Arnold A, et al., New Eng J Med 1983; 309:1593-1599. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of human tonsil tissue labelling IgD with BP6134. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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