CD20 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50134 Clone# BP6138 Predicted Molecular Wt: 33kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P11836 Background: CD20 is a membrane-embedded 33kDa nonglycosylated phosphoprotein which play a role in B-cell activation, differentiation, and cell-cycle progression. CD20 is a restricted B-cell antigen and its clinical utility is greatest for identifying B-lineage non- Hodgkin’s lymphomas, but it can also aid in the diagnosis of nodular lymphocyte predominance Hodgkin’s disease and acute lymphoblastic neoplasms. Subcellular location: Membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to CD20 residues within aa197‐C terminal of CD20 was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. N Johnson., et al. Blood 2009 113:3773-3780. 2. Clark EA., et al. Proc Natl Acad Sci U S A 1985;82:1766-1770. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Tonsil tissue labelling CD20 with BP6138. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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