PAX-5 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50136 Clone# BP6140 Predicted Molecular Wt: 42kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: Q02548 Background: Pax genes area family of developmental control genes that encode nuclear transcription factors and have been implicated in the control of mammalian development. PAX-5 is a marker for B-cells, including B-lymphoblastic neoplasms and maturation stage. It is found in most cases of mature and precursor B-cell non-Hodgkin lymphomas/leukemias. In approximately 97% of cases of classic Hodgkin lymphoma, Reed- Sternberg cells express PAX-5. The antibody is a useful tool for the identification of pro, pre, and mature B cells and for classification of lymphomas and subclassification of classic Hodgkin’s lymphoma and anaplastic large cell lymphoma of the T and null-cell type. Subcellular location: Nucleus Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to residues within aa300 to aa400 of PAX-5 was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Abdelrasoul H etal. Sci Rep 8:1327 (2018). 2. Hijano DR etal. Sci Rep 8:11034 (2018) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of tonsil labelling PAX-5 with BP6140. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
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