Desmin兔单克隆抗体

价格¥600
规格100ul
货号HKZ150262
单位支
品牌HaoKebio

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品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150262	Desmin兔单克隆抗体	100ul	支	600

Desmin
Mouse Monoclonal Antibody Product Datasheet
Catalog# BX50139 Clone# D33
Predicted Molecular Wt:
53kDa
Purity:   ProG affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P17661
Background:
Desminis a 53kDa intermediate filament (IF) protein that integrates the sarcolemma, Z disk, and nuclear membrane in
sarcomeres and regulates sarcomere architecture. Desmin play no role in contractility but serves to maintain the orientation of actin and myosin filaments and may also play a role in nuclear transcription.
Desminis expressed in all striated muscle cells and most smooth muscle cells. In the developing striated muscle cells it replaces vimentin, linked to the Z-disc. In the smooth muscle cells desminis associated with cytoplasmic dense bodies and
subplasmalemmal dense plaques. In myofibroblasts and vascular smooth muscle cells desminis co-expressed with vimentin.
However, in the arterial smooth muscle cells, desmin may be scarce or absent, while vimentin expression is retained. Mesothelial cells may express desmin, particularly in effusions and cultures. In tumor tissues, it detected in leiomyomas, leiomyosarcoma,
rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.
Desminis used in a panel for identification of leiomyosarcoma, rhabdomyosarcoma and other tumours with myoid differentiation, and for classification of mesenchymal, pleomorphic, spindle cell and round cell neoplasms.
Subcellular location:
Cytoplasm
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Immunogen derived from human Leiomyoma
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Brodehl A., et al. Circ Cardiovasc Genet. 2013 Dec;6(6):615-23.
2. Colella R., et al. Am J Surg Pathol. 2010 Jan;34(1):10-7.
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections) analysis of
esophagus tissue labelling Desmin with D33. Heat
mediated antigen retrieval was performed using
Tris/EDTA buffer pH 9.0

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