CD15鼠单克隆抗体

CD15鼠单克隆抗体

  • 价格¥600
  • 规格100ul
  • 货号HKZ150263
  • 单位
  • 品牌HaoKebio

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品牌商品编码商品名称规格单位单价
HaoKebioHKZ150263CD15鼠单克隆抗体100ul600
CD15
Mouse Monoclonal Antibody Product Datasheet
Catalog#  BX50140 Clone#  MMA
Predicted Molecular Wt:
59kDa
Purity:  Affinity purified IgM
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P22083
Background:
CD15 is present on greater than 90% of granulocytes including neutrophils and eosinophils, and to a lesser degree, on monocytes. CD15 is expressed in Reed-Sternberg cells of Hodgkin’s disease (of the nodular sclerosis, mixed cellularity and lymphocyte-
depleted subtypes), and certain types of epithelial cells. It is generally agreed that the Reed-Sternberg cell variants in lymphocyte- predominant Hodgkin’s disease are not reactive with CD15. Positive staining for CD15 combined with a negative reaction for
lymphocytic markers may provide support for Hodgkin’sdisease.
Subcellular location:
Membrane
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer
(pH 9.0), primary antibody incubate at RT (18°C-25°C)
for 30 minutes.
Immunogen:
Immunized with the U-937 histiocytic cell line
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA
0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store
at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1.  Pellegrini W, et al. Haematologica. 2007 May;
92(5):708-9.
2. Song JY, et al. Am J Surg Pathol. 2011 May; 35(5):767- 72.
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections) analysis of
Hodgkin’s Lymphoma
tissue labelling CD15 with
MMA. Heat mediated
antigen retrieval was
performed using Tris/EDTA
buffer pH 9.0

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