Actin (Smooth Muscle) Mouse Monoclonal Antibody Product Datasheet Catalog# BX50141 Clone# 1A4 Predicted Molecular Wt: 42kDa Purity: ProG affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P62736 Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Invertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and area major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Subcellular location: Cytoplasm Recommended method: Don’t need to repair. Immunogen: N-terminal synthetic decapeptide of Human α-smooth muscle actin Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. GabbianiG,et.al, Proc Natl Acad Sci U S A. 1981 Jan;78(1):298-302. 2. Itoga T, et.al,Pathol Int. 2003 Jun;53(6):371-5. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of cervical leiomyoma tissue labelling Mouse Monoclonal Antibody with 1A4.
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