Caldesmon Mouse Monoclonal Antibody Product Datasheet Catalog# BX 50166 Clone# h-CALD Predicted Molecular Wt: 93.2kDa Purity: ProG affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: Q05682 Background: Anti-caldesmon is a regulatory protein found in smooth muscle and other tissues which interacts with actin, myosin, tropomyosin, and calmodulin. Anti-caldesmon antibody labels smooth muscle and tumors of smooth muscle, myofibroblastic, and myoepithelial differentiation. Anti-caldesmon has also been used to differentiate epithelioid mesothelioma from serous papillary carcinoma of the ovary. Subcellular location: Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to Caldesmon was used as an immunogen. The exact sequence is proprietary. Storage conditions: 2°C-8°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at 2°C-8°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Nakagaki-Silva EE etal. Elife 8: (2019). 2. Domke LM . Cell Tissue Res (2018). Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Colon tissue labelling Caldesmon with h-CALD. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
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