NSE Mouse Monoclonal Antibody Product Datasheet Catalog# BX50169 Clone# BPM6150 Predicted Molecular Wt: 47kDa Purity: ProG affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P09104 Background: NSE is an intracytoplasmic protein, and enolase is a dimer in mammals. It has three subunits: 1, 2, and 3, which are mainly distributed in central nerve, peripheral nerve, neuroendocrine cells and tumor cells.This antibody can specifically recognize 47kDa of NSE, and can be used for the auxiliary diagnosis of neuroendocrine tumors. Due to the poor specificity of the antibody, some normal smooth muscle, myoepithelial cells, renal tubular cells, lymphocytes can also express NSE. Therefore, this antibody is not used as a single diagnostic marker, and should be applied in combination with other indicators to improve its diagnostic accuracy. Subcellular location: Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide within Human NSE aa 416-433. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Thrivikraman G et al. Nat Commun 10:3520 (2019). 2. Foucar, K et al. Int J Lab Hematol. 2020;00:1–5. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human neuroblastoma tissue labelling NSE with BPM6150. Heat mediated antigen retrieval was performed using Tris/EDTA buffer
杭州浩克生物
您好！请登录