IgG Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50183 Clone# BP6161 Predicted Molecular Wt: 36kD Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P55899 Background: Immunoglobulin G (IgG) is an antibody isotype secreted by plasma cells and composed of four peptide chains – two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. In humans, IgG consists of four subclasses that differ only marginally in their amino acid composition. Representing approximately 75% of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. Anti-IgG had been proven useful in the assessment of renal biopsies, autoimmune disorders, in the identification of plasma cell neoplasms and in non-Hodgkin lymphomas. The ratio of IgG4+ plasma cells to IgG+ plasma cells has been considered important in making a diagnosis of IgG4- related disorders. Subcellular location: Cytoplasm Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Full length native protein (purified) corresponding to Human Human IgG. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Leong AS, et al. Geenwich Medical Media Ltd. 1999; 217-219. 2. Ando K, et al. Intern Med. 2000; 39:170-5. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections analysis of human tonsil tissue labelling IgG with BP6161. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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