Oct-3/4兔单克隆抗体

价格¥1000
规格100ul
货号HKZ150309
单位支
品牌HaoKebio

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品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150309	Oct-3/4兔单克隆抗体	100ul	支	1000

Oct-3/4
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# BX50186 Clone# BP6164
Predicted Molecular Wt:
38.5kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:  Q01860
Background:
OCT-4, also known as OTF3 or POU5F1, is a member of the POU family of transcription factors, involved in the regulation of pluripotency during normal development and is detectable in embryonic stem and germ cells. It can specifically bind to the  octamer motif (5′-ATTTCAT-3′), and it is critical for the self-renewal of embryonic stem cells. OCT-4 is a key regulator of self- renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis and can affect some aspects of   tumor behavior such as tumor recurrence or resistance to therapies.  OCT-4 is a sensitive and specific marker for germ cell    tumors. It is consistently detected in carcinoma in situ/gonadoblastoma, seminomas, germinoma, dysgerminoma, and
embryonal carcinoma but not in the differentiated components of nonseminomas. An antibody to OCT-4 is useful in the identification of primary as well as metastatic germ cell tumors.
Subcellular location:
Nucleus
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to residues in Human Oct-3/4 was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Zhang S  et al. Nucleic Acids Res 47:4449-4461 (2019).
2. Chang YC  et al. Cell Death Dis 11:195 (2020).
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded
sections) analysis
of  human seminoma
tissue labelling Oct-3/4 with BP6164. Heat
mediated antigen
retrieval was performed using Tris/EDTA buffer    pH 9.0

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