FOXP3 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50188 Clone# BP6166 Predicted Molecular Wt: 47kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: Q9BZS1 Background: Forkhead box (Fox) proteins area family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain. The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 – FoxP4, which are broadly expressed and play important roles in organ development, immune response, and cancer pathogenesis. FoxP proteins usually function as transcriptional repressors. FOXP3 is expressed in activated T lymphocytes in tonsil in adult, T cell leukemia or lymphoma. Subcellular location: Nucleus Recommended Method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Recombinant fragment corresponding to human FOXP3 was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage Conditions: -25°C to -18°C Shipment Instructions: Shipped on blue ice. Upon delivery store at -25°C to -18 °C. Avoid freeze / thaw cycles. Recommended Dilution: IHC-P: 1:100-1:200 Background References: 1. Zuo, T. et al. (2007) Cell 129, 1275-86. 2. Wang, L. et al. (2009) Cancer Cell 16, 336-46. Immunohistochemistry (Formalin/PFA-fixed paraffin- embedded sections) analysis of tonsil labelling FOXP3 with BP6166.
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