O lig2兔单克隆抗体

O lig2兔单克隆抗体

  • 价格¥600
  • 规格100ul
  • 货号HKZ150312
  • 单位
  • 品牌HaoKebio

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HaoKebioHKZ150312O lig2兔单克隆抗体100ul600
Olig2
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog#  BX50189 Clone#  BP6167
Predicted Molecular Wt:
32kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:  Q13516
Background:
Olig2, a basic helix–loop–helix transcription factor, is involved in oligodendroglial specification. Olig2 expression has been
reported in most glial tumors, such asoligodendrogliomas and astrocytomas. Although more than half of glioblastomas are
positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has    been found in the non-glial tumors including neuroepithelial tumors, ependymomas, subependymomas, medulloblastomas,     and nonneuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid/rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral  brain tissue.
Subcellular location:
Nucleus
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide corresponding to residues in Human Olig2 was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Shou JW  etal. Front Cell Neurosci 13:395 (2019).
2. Cho KHT  etal. Sci Rep 10:6904 (2020).
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded
sections) analysis
of  human brain tissue labelling Olig2 with
BP6167. Heat mediated antigen retrieval was
performed using
Tris/EDTA buffer pH 9.0

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