TFE3兔单克隆抗体

价格¥600
规格100ul
货号HKZ150203
单位支
品牌HaoKebio

产品介绍

产品参数

资料下载

品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150203	TFE3兔单克隆抗体	100ul	支	600

TFE3
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# HKZ150203 Clone# BP6180
Predicted Molecular Wt:
62kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P19532
Background:
Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. Specifically recognizes and binds E-box sequences; efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such
as TFEB or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation. Upon starvation or
lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in nuclear localization and transcription factor activity . In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent
antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity . Specifically recognizes the MUE3
box,a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site. Acts as a positive regulator of browning of adipose tissue by promoting expression of target genes; mTOR-dependent phosphorylation
promotes cytoplasmic retention of TFE3 and inhibits browning of adipose tissue. Maintains the pluripotent state of embryonic stem cells by promoting the expression of genes such as ESRRB; mTOR-dependent nuclear exclusion promotes exit from
pluripotency . Required to maintain the naive pluripotent state of hematopoietic stem cell; mTOR-dependent cytoplasmic retention of TFE3 promotes the exit of hematopoietic stem cell from pluripotency.
Subcellular location:
Nucleus
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Miller, A.J. et al. (2005) J Biol Chem 280, 146-55
2. Kauffman, E.C. et al. (2014) Nat Rev Urol 11, 465-75.
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections) analysis of Renal cell carcinoma labelling
TFE3 with BP6180. Heat
mediated antigen retrieval was performed using
Tris/EDTA buffer pH 9.0.

HKZ150203_BP6180_TFE3-说明书下载

暂无