Tim-3 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# HKZ150205 Clone# BP6182 Predicted Molecular Wt: 33kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: Q8TDQ0 Background: T cell Ig- and mucin-domain-containing molecules (TIMs) area family of transmembrane proteins expressed by various immune cells. TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer, and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype. TIM-3 is an inhibitory molecule that is induced following T cell activation.Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells. Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced antitumor responses in a mouse model of colorectal cancer. In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease. Subcellular location: Membrane Recommended method: Heat induced epitope retrieval with Tris EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide corresponding to Human Tim-3 protein was used as an immunogen. The exact sequence is proprietary. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Monney, L. et al. (2002) Nature 415, 536-41. 2. Chiba, S. et al. (2012) Nat Immunol 13, 832-42. 3.Huang, Y.H. et al. (2015) Nature 517, 386-90. Immunohistochemistry (Formalin/PFA-fixed paraffin-embeddedsections) analysis of tonsillabelling Tim-3 withBP6182. Heat mediatedantigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
您好！请登录