LAG-3 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# HKZ150215 Clone# BP6192 Predicted Molecular Wt: 57kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P18627 Background: Lymphocyte activation gene 3 (LAG-3, CD223) is an immune checkpoint control protein that negatively regulates T cells and immune responses. A CD4-like member of the Ig superfamily, LAG3 contains an extracellular IgVand three IgC domains, a transmembrane domain, and a short cytoplasmic region. LAG3 is primarily expressed by activated CD4+ T cells, CD8+ T cells, Tregs and NK cells, where it’s activated by MHC Class II molecules, its only known ligand. While it was initially shown to activate Treg cells, LAG3 can also inhibit CD8+ T cells. LAG3 is often co-expressed with PD-1 on the surface of tumor infiltrating lymphocytes, where the two proteins act independently to contribute to tumor-mediated immune suppression. Blockade of LAG3 is a promising strategy for neoplastic intervention. Subcellular location: Membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide within Human LAG-3. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Matsuzaki, J. et al. (2010) Proc Natl Acad Sci U S A 107, 7875-80. 2. Woo, S.R. et al. (2012) Cancer Res 72, 917-27. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tis sue labelling LAG-3 with BP6192. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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