UroplakinIII Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50222 Clone# BP6199 Predicted Molecular Wt: 30kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: O75631 Background: Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. It also contributes to the formation of urothelial glycocalyx which may play an important role in preventing bacterial adherence. Subcellular location: Cytoplasm/Membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Full length protein corresponding to Hu Uroplakin Ⅲ. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Lobban E.D., Smith B.A., Hall G.D., Harnden P., Roberts P., Selby P.J., Trejdosiewicz L.K., Southgate J.Am. J. Pathol. 153:1957-1967 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of urothelial carcinom tissue labelling UroplakinⅢ with BP6199. Heat mediated antigenretrieval was performed using Tris/EDTA buffer
杭州浩克生物
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