COL4A5 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50229 Clone# BP6206 Predicted Molecular Wt: 161kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P29400 Background: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a ‘chicken-wire’ meshwork together with laminins, proteoglycans and entactin/nidogen. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Subcellular location: basement membrane Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Recombinant fragment with COL4A5. This information is proprietary to Biolynx and/or its suppliers. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Tsugio Seki et al. Histochem Cell Biol (1998) 110:359-366 2. Yoshioka K etal. Am. J. Pathol (1994) . 144: 986-996 Immunohistochemistry (Formalin/PFA-fixed paraffin- embedded sections) analysis of human kidney tissue labelling COL4A5 with BP6206. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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