Bcl-6 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# HKZ150239 Clone# BP6216 Predicted Molecular Wt: 79kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P41182 Background: Bcl-6 is a transcriptional regulator gene which codes for a 706-amino-acid nuclear zinc finger protein.The mitogen-activated protein kinases, Erk1 and Erk2, but not JNK, phosphorylate BCL6 at multiple sites. Phosphorylation of BCL6 at Ser333 and Ser343 results in degradation of BCL6 by the ubiquitin/proteasome pathway in B cells. In addition, BCL6 is acetylated and its transcriptional repressor function is inhibited by the transcriptional co-activator p300. Antibodies to this protein stain the germinal center cells in lymphoid follicles, follicular cells and interfollicular cells in Follicular Lymphoma, Diffuse Large B-Cell Lymphomas, Burkitt’s Lymphoma, and the majority of the Reed-Sternberg cells in Nodular Lymphocyte-Predominant Hodgkin’s Disease. Subcellular location: Nuclear Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Recombinant fragment within Human Bcl-6. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Shvarts, A. et al. (2002) Genes Dev 16, 681-6. 2. Bereshchenko, O.R. et al. (2002) Nat Genet 32, 606-13. Immunohistochemistry (Formalin/PFA-fixed paraffin- embedded sections) analysis of human diffuse large B cell lymphoma tissue labelling Bcl-6 with BP6216. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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