MGMT Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# HKZ150240 Clone# BP6217 Predicted Molecular Wt: 22kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P16455 Background: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to acysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Subcellular location: Nucleus Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: A synthetic peptide corresponding to residues on the N-terminus of human MGMT was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Bobola, M.S. et al. (2005) Clin. Cancer Res. 11, 2747-2755. 2. Pollack, I.F. et al. (2006) J. Clin. Oncol. 24, 3431-3437. Immunohistochemistry (Formalin/PFA-fixed paraffin- embedded sections) analysis of human endometrial cancer tissue labelling MGMT with BP6217. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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