MGMT兔单克隆抗体

价格¥1400
规格100ul
货号HKZ150363
单位支
品牌HaoKebio

产品介绍

产品参数

资料下载

品牌	商品编码	商品名称	规格	单位	单价
HaoKebio	HKZ150363	MGMT兔单克隆抗体	100ul	支	1400

MGMT
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# BX50240 Clone# BP6217
Predicted Molecular Wt:
22kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:   P16455
Background:
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to acysteine residue in the enzyme. This is a
suicide reaction: the enzyme is irreversibly inactivated.
Subcellular location:
Nucleus
Recommended method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
A synthetic peptide corresponding to residues on the
N-terminus of human MGMT was used as an immunogen.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage conditions:
-25°C to -18°C
Storage instructions:
Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles.
Recommended Dilutions:
IHC-P:     1:100-1:200
Background References:
1. Bobola, M.S. et al. (2005) Clin. Cancer Res. 11, 2747-2755.
2. Pollack, I.F. et al. (2006) J. Clin. Oncol. 24, 3431-3437.
Immunohistochemistry
(Formalin/PFA-fixed paraffin-
embedded sections) analysis of     human endometrial cancer tissue labelling MGMT with BP6217.
Heat mediated antigen retrieval was performed using Tris/EDTA  buffer pH 9.0

HKZ150363_BP6217_MGMT 下载

暂无