Brachyury Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50241 Clone# BP6218 Predicted Molecular Wt: 47kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: O15178 Background: Brachyury protein, encoded by the T gene, is a transcription factor that is vital for the formation of posterior mesoderm and axial development during vertebrate embryogenesis. Human brachyury is expressed in the notochord, as well as in chordoma tumors that occur along the spine, making it a good marker for notochord and notochord-derived tumors. A common polymorphism in the human T gene has also been shown to be associated with development of the multifactorial neural tube defect, spina bifida. Subcellular location: Nuclear Recommended method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Recombinant fragment within Human Brachyury. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage conditions: -25°C to -18°C Storage instructions: Shipped on blue ice. Upon delivery, aliquot, and store at -25°C to -18°C. Avoid freeze / thaw cycles. Recommended Dilutions: IHC-P: 1:100-1:200 Background References: 1. Vujovic, S. et al. (2006) J Pathol 209, 157-65. 2. Yang, X.R. et al. (2009) Nat Genet 41, 1176-8. Immunohistochemistry (Formalin/PFA-fixed paraffin- embedded sections) analysis of human chordoma tissue labelling Brachyury with BP6218. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0
杭州浩克生物
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