HBsAg Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50247 Clone# BP6224 Predicted Molecular Wt: 42kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P03142 Background: Hepatitis B stool surface antigen (HBsAg) is a glycoprotein on the surface of hepatitis B virus. After infection with HBV, HBsAg appears as first virus marker. HBsAg can be found in the blood, saliva, breast milk, sweat, tears, nasopharyngeal secretions, semen and vaginal secretions of patients 2 to 6 months after infection with hepatitis B virus, and 2 to 8 weeks before the elevation of alanine aminotransferase, a positive result can be detected in the serum. HBsAg antibody is mainly used in the diagnosis of hepatitis B virus. Subcellular location: Cytoplasm Recommended Method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Recombinant fragment within Human HBsAg was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage Conditions: -25°C to -18°C Shipment Instructions: Shipped on blue ice. Upon delivery store at -25°C to -18 °C. Avoid freeze / thaw cycles. Recommended Dilution: IHC-P: 1:100-1:200 Background References: 1. Amano H, et al. (2021) Viruses 13, 2216. 2. Salpini R, et al. (2015) Hepatology 3, 823-833. Immunohistochemistry (Formalin/PFA-fixed paraffin- embedded sections) analysis of HBV infected liver labelling HBsAg with BP6224.
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