CDK4 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50253 Clone# BP6230 Predicted Molecular Wt: 34kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: P11802 Background: Cyclin-dependent kinase-4 (CDK4) is a protein-serinekinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level (PMID:17253961). CCND1- CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth (PMID:21844184). CDK4 is highly expressed in highly differentiated and dedifferentiated liposarcomas, but rarely expressed in other benign liposarcomas and other sarcomas. CDK4 and MDM2 combined to differentiate between highly differentiated liposarcoma (+), dedifferentiated liposarcoma (+) and myxoid liposarcoma, pleomorphic liposarcoma, spindle lipoma, pleomorphic lipoma and other high-grade sarcomas. Subcellular location: Nucleus Recommended Method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: A synthetic peptide corresponding to the N-term of CDK4 was used as an immunogen. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage Conditions: -25°C to -18°C Shipment Instructions: Shipped on blue ice. Upon delivery store at -25°C to -18 °C. Avoid freeze / thaw cycles. Recommended Dilution: IHC-P: 1:100-1:200 Background References: 1. Ma L etal. Oncotarget 8:4125-4135 (2017). 2. Lv XJ et al. Oncol Rep N/A:N/A (2016). Immunohistochemistry (Formalin/PFA-fixed paraffin- embedded sections) analysis of dedifferentiated liposarcoma labelling CDK4 with BP6230.
杭州浩克生物
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