COL4A3 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# HKZ150277 Clone# BP6254 Predicted Molecular Wt: 162kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: Q01955 Background: Six α chain of Type IV collagen can aggregate into 3 triple helix molecular structure, which called monomer, monomer polymerization to form adimer or tetramer, which can twist each other to form a collagen network structure. Mutations in the X-linked dominant Alport comprehensive acquisition gene mainly occurred in the gene encoding collagen type IV ɑ5 chain (COL4A5). Mutations in the autosomal recessive Alport syndrome occur on chromosome 2 in genes encoding collagen type IV ɑ3 or ɑ4 strands (COL4A3/COL4A4) .COL4A3 is mainly expressed in podocytes in Bowman’s capsule of glomeruli. Subcellular location: Basement membrane Recommended Method: High temperature and high pressure induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Amplifier for Mouse + Rabbit IgG for 25min ， HRP Polymer Detector for 25 min. Immunogen: Recombinant protein fragment corresponding to human COL4A3. This information is proprietary to Biolynx and/or its suppliers. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage Conditions: -25°C to -18°C Shipment Instructions: Shipped on blue ice. Upon delivery store at -25°C to -18 °C. Avoid freeze / thaw cycles. Recommended Dilution: IHC-P: 1:100-1:200 Background References: 1. Tsugio Seki et al. Histochem Cell Biol (1998) 110:359- 366 2. Yoshioka K etal. Am. J. Pathol (1994) . 144: 986-996 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney labelling COL4A3 with BP6254.
杭州浩克生物
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