E2F4 Recombinant Rabbit Monoclonal Antibody Product Datasheet Catalog# BX50279 Clone# BP6256 Predicted Molecular Wt: 44kDa Purity: ProA affinity purified IgG Species Cross-reactivity: Human Form: Liquid Applications: IHC-P Swissprot ID: Q16254 Background: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5′-TTTC[CG]CGC-3′ found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein. E2F4 was considered a potential prognostic factor for HNSCC. Immunohistochemical staining showed that E2F4 was mainly localized in the cell nucleus; it was highly expressed in HNSCC tissues, with a significant difference noted from that in pericancerous mucosa tissues. Subcellular location: Nucleus Recommended Method: Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. Immunogen: Synthetic peptide. This information is proprietary to Biolynx. Storage Buffer: PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. Storage Conditions: -25°C to -18°C Shipment Instructions: Shipped on blue ice. Upon delivery store at -25°C to -18 °C. Avoid freeze / thaw cycles. Recommended Dilution: IHC-P: 1:100-1:200 Background References: 1. I Molina-Privado1, et al. Leukemia (2012) 26, 2277- 2285. 2. Li Qi, et al. Scientifc Reports(2022) 12:12132. Immunohistochemistry (Formalin/PFA-fixed paraffin- embedded sections) analysis of human breast cancer labelling E2F4 with BP6256.
杭州浩克生物
您好！请登录