E2F4兔单克隆抗体

E2F4兔单克隆抗体

  • 价格¥2200
  • 规格100ul
  • 货号HKZ150279
  • 单位
  • 品牌HaoKebio

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品牌商品编码商品名称规格单位单价
HaoKebioHKZ150279E2F4兔单克隆抗体100ul2200
E2F4
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog#  HKZ150279 Clone#  BP6256
Predicted Molecular Wt:
44kDa
Purity:   ProA affinity purified IgG
Species Cross-reactivity:
Human
Form:   Liquid
Applications:
IHC-P
Swissprot ID:  Q16254
Background:
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5′-TTTC[CG]CGC-3′ found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.
E2F4 was considered a potential prognostic factor for HNSCC. Immunohistochemical staining showed that E2F4 was mainly localized in the cell nucleus; it was highly expressed in HNSCC tissues, with a significant difference noted from that in pericancerous mucosa tissues.
Subcellular location:
Nucleus
Recommended Method:
Heat induced epitope retrieval with Tris-EDTA buffer   (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide. This information is proprietary to Biolynx.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage Conditions:
-25°C to -18°C
Shipment Instructions:
Shipped on blue ice. Upon delivery store at -25°C to -18 °C. Avoid freeze / thaw cycles.
Recommended Dilution:
IHC-P:     1:100-1:200
Background References:
1.  I Molina-Privado1, et al. Leukemia (2012) 26, 2277- 2285.
2.  Li Qi, et al. Scientifc Reports(2022) 12:12132.
Immunohistochemistry (Formalin/PFA-fixed paraffin-  embedded sections) analysis of human breast cancer labelling E2F4 with BP6256.

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