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| 品牌 | 商品编码 | 商品名称 | 规格 | 单位 | 单价 |
|---|---|---|---|---|---|
| HaoKebio | HKZ150307 | PLA2R兔单克隆抗体 | 100ul | 支 | 1000 |
Recombinant Rabbit Monoclonal Antibody Product Datasheet
Catalog# HKZ150307 Clone# BP6284
Predicted Molecular Wt:
169kDa
Purity: ProA affinity purified IgG
Species Cross-reactivity:
Human
Form: Liquid
Applications:
IHC-P
Swissprot ID: Q13018
Background:
M-type phospholipase A2 receptor(PLA2R) , acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-
IIA/PLA2G2A. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In podocytes, binding of sPLA2-IB/PLA2G1B can regulate podocyte survival and glomerular homeostasis.
PLA2R is highly expressed in podocytes in human glomeruli, also expressed in pancreas, amnion, choriodecidua and placenta.
PLA2R was identified as a major target antigen involved in idiopathic membranous nephropathy in adults. Circulating
autoantibodies against PLA2R were found in 70 to 80% of patients with idiopathic membranous nephropathy . It has been suggested that the serum level of PLA2R autoantibody could be used for the diagnosis of idiopathic membranous
nephropathy and for the monitoring of response to treatment.
Subcellular location:
Membranous
Recommended Method:
Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes.
Immunogen:
Synthetic peptide. This information is proprietary to Biolynx.
Storage Buffer:
PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.
Storage Conditions:
-25°C to -18°C
Shipment Instructions:
Shipped on blue ice. Upon delivery store at -25°C to – 18°C. Avoid freeze / thaw cycles.
Recommended Dilution:
IHC-P: 1:100-1:200
Background References:
1. Beck LH Jr, et al. Kidney Int 77:765-770, 2010.
2. R.J. Glassock.Contrib Nephrol, 181:131-142,2013.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glomeruli tissue sections labeling
PLA2R with BP6284.
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